Experimental research techniques course - module B
This module is of interest to those who want to master experimental research techniques on small rodents using the operating microscope’s power and basic microsurgical techniques. If appropriate for you this can include:
- pharmacology models, for infusion and blood sampling, temporary occlusion stroke models, for transplantation models
- focal and global ischemia models in rats and mice
- cardio-vascular models (MI and TAC) or any others specifically relevant to your needs
- pharmacokinetic models, for infusion and blood sampling from arteries, veins, bile duct, gut or any other places relevant to your needs
- toxicology models, for a single dose, multiple-dose acute and chronic study designs and performance
- different techniques of particular interest to participants by agreement before or during the module
Trainings are provided at Almere in The Netherlands. Ask us about training dates!
Day 1: Basic microsurgical techniques
- 09.00 - 09.30 Welcome, introduction and expectations for the course
- 09.30 - 10.45 Good Surgical Practice (GSP) in the rat
- 10.45 - 11.00 Coffee and tea
- 11.00 - 11.45 Instruments
- 11.45 - 13.00 Suture materials and suture techniques
Practical exercises:
- 14.00 - 15.30 Microsurgical suture techniques on a latex glove
- 15.30 - 17.00 Anastomoses techniques on artificial vessels, using the MD PVC-Rat
Day 2: Experimental microsurgical techniques I
- 09.00 - 09.30 Vascular access
- 09.30 - 10.15 Jugular vein catheterization (stress-free blood sampling)
- 10.15 - 10.30 Coffee and tea
Practical exercises:
- 10.30 - 12.30 Jugular vein catheterization
- 13.30 - 14.00 End-to-end anastomosis of the rat femoral artery (theory)
- 14.00 - 17.00 Dissection techniques and vascular anastomosis (rat femoral artery)
Day 3: Experimental microsurgical techniques II
- 09.00 - 10.15 Anaesthesia including peri-operative care
- 10.15 - 10.30 Coffee and tea
Practical exercises:
- 10.30 - 17.00 End-to-side anastomosis of an artery. End-to-end anastomosis of a vein
Day 4: Experimental microsurgical techniques III
- 09.00 - 10.00 Asepsis
- 10.00 - 11.00 Catheterization of vessels and ducts
Practical exercises:
11.00 - 17.00: Catheterization of:
- Portal vein
- Carotid artery
- Bile duct (intact EHC)
- Femoral vein
Day 5: Experimental microsurgical techniques IV
- 09.00 - 10.00 Discussion on requested techniques
- 10.00 - 10.15 Coffee and tea
Practical exercises:
- 10.15 - 16.00 Techniques of request
- 16.00 - 16.15 Course evaluation
Organizational information
Each participant has an own set-up with operating microscope, all (micro)surgical tools,
suture materials and pathogen free laboratory rats and mice required for the course. The number of attendees
per course is limited to 8 participants.
The registration fee includes the following items:
- A numbered certificate
- Hand-out of all PowerPoint slides used
- Documentation on the surgical tools, catheters and operating microscopes used
- Lunches, coffee, tea and soft drinks
