Experimental research techniques and radio-telemetry in small rodents, course, module C 5-days
Radio-telemetry is a low postsurgical stress method to measure physiological parameters such as heart rate, electrocardiogram (ECG), blood pressure (BP), ventricular pressures (LVP and RVP) blood flow, acceleration and body temperature in freely moving laboratory animals. Let's join to 5-days surgical training in Almere in The Netherlands. Ask us about training dates!
Day 1: Basic microsurgical techniques
- 09.00 - 09.30 Welcome, introduction and expectations for the course
- 09.30 - 10.45 Good Surgical Practice (GSP)
- 10.45 - 11.00 Coffee and tea
- 11.00 - 11.45 Instruments
- 11.45 - 13.00 Suture materials and suture techniques
Practical exercises:
- 14.00 - 15.30 Microsurgical suture techniques on a latex glove
- 15.30 - 17.00 Anastomoses techniques on artificial vessels, using the MD PVC-Rat
Day 2: Experimental microsurgical techniques
- 09.00 - 09.30 Vascular access
- 09.30 - 10.15 Jugular vein catheterization (stress-free blood sampling)
- 10.15 - 10.30 Coffee and tea
Practical exercises:
- 10.30 - 12.30 Jugular vein catheterization
- 13.30 - 14.00 End-to-end anastomosis of the rat femoral artery (theory)
- 14.00 - 17.00 Dissection techniques and vascular anastomosis (rat femoral artery)
Day 3: Experimental techniques, Radio-telemetry I
- 09.00 - 10.15 Anaesthesia including peri-operative care
- 10.15 - 10.30 Coffee and tea
- 10.30 - 11.00 Radio-telemetry, past, present and future. Tips and tricks for BP/ECG implantation
Practical exercises:
- 11.00 - 17.00 Implantation ECG/BP module, carotid artery or the abdominal aorta (rat or mouse)
Day 4: Experimental techniques, Radio-telemetry II
- 09.00 - 09.30 Asepsis
Practical exercises:
- 10.00 - 17.00: Implantation telemetry device of choice (rat or mouse)
Day 5: Experimental techniques, Radio-telemetry III
- 09.00 - 09.30 Inventory of requested techniques
- 09.30 - 10.00 Discussion on technical approaches and refinement
Practical exercises:
- 10.00 - 16.00 Requested techniques
- 16.00 - 16.30 Closing remarks and course evaluation
Organizational information
Each participant has an own set-up with operating microscope, all (micro)surgical tools, suture materials and pathogen free laboratory rats and mice required for the course. The number of attendees per course is limited to 8 participants.
The registration fee includes the following items:
- A numbered certificate
- Hand-out of all PowerPoint slides used
- Documentation on the surgical tools, catheters and operating microscopes used
- Lunches, coffee, tea and soft drinks